Journal: Cell reports

Article Title: GluN3A subunit tunes NMDA receptor synaptic trafficking and content during postnatal brain development

doi: 10.1016/j.celrep.2023.112477

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-GluN2A , Merck Millipore , Cat# M264.

Techniques: Cell Culture, Recombinant, Software

Journal: Frontiers in Neuroscience

Article Title: Altered Light Sensitivity of Circadian Clock in Shank3 +/– Mouse

doi: 10.3389/fnins.2021.604165

Figure Lengend Snippet: Immunostaining for cholera toxin β-subunit (CTB) and NMDAR2A in the suprachiasmatic nucleus (SCN) from wild-type (WT) and Shank3 +/– mice. CTB (diaminobenzidine, DAB, left column ) and NMDAR2A ( right column ) did not show obvious differences in the immunoreactivity between groups (WT, top micrographs ; Shank3 +/– , bottom micrographs ). For this and subsequent figures ( , ), scale bar = 50 μm.

Article Snippet: Primary antibodies were diluted as indicated in 0.1 M PBS containing 1.0% normal serum in 0.3% Triton X-100 [anti-VIP raised in rabbit, CAT 20077, Incstar, 1:2,000 ( ); anti-CTB subunit raised in goat, CAT 703, List Biological Laboratories, 1:2,000 ( ); anti-NMDAR2A (GluN2A) raised in rabbit, CAT ACG 002, Alomone Labs, 1:400 ( ); and anti c-FOS raised in rabbit, CAT SC-52, Santa Cruz, 1:1,000 ( )].

Techniques: Immunostaining

Journal: Molecular Neurobiology

Article Title: The Golgi-Associated PDZ Domain Protein Gopc/PIST Is Required for Synaptic Targeting of mGluR5

doi: 10.1007/s12035-021-02504-9

Figure Lengend Snippet: Primary antibodies. WB , dilution for Western blotting; IF , dilution for immunofluorescence staining

Article Snippet: GluN2A , Rabbit , WB 1:1000 , 5% milk powder/TBS-T , Novus Biologicals (NB300-105).

Techniques: Western Blot, Immunofluorescence

Journal: Translational Cancer Research

Article Title: Ovarian teratomas causing anti-N-methyl-D-aspartate receptor encephalitis: a case series from west China

doi: 10.21037/tcr-24-2126

Figure Lengend Snippet: Expression of NMDAR staining in the squamous epithelium and neural tissue of different groups. (A) NMDAR2B staining in the squamous epithelium (the arrow points to the squamous epithelium) was strongly positive in the encephalitis group. (B) NMDAR2A staining in the squamous epithelium (the arrow points to the squamous epithelium) was moderately positive in the encephalitis group. (C) NMDAR1 staining in the squamous epithelium (the arrow points to the squamous epithelium) was moderately positive in the encephalitis group. (D) NMDAR2B staining in the neural tissue was moderately positive in the only case of encephalitis group. (E) NMDAR2A staining in the neural tissue was moderately positive in the only case of encephalitis group. (F) NMDAR1 staining in the neural tissue was slightly positive in the only case of encephalitis group. (G) NMDAR2B staining in the neural tissue or squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the IMT group. (H) NMDAR2A staining in the neural tissue or squamous epithelium (as the arrow pointed) was moderately positive in a few cases of the IMT group. (I) NMDAR1 staining in the neural tissue or squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the IMT group. (J) NMDAR2B staining in the squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the MT group. (K) NMDAR2A staining in the squamous epithelium (as the arrow pointed) was moderately positive in a few cases of the MT group. (L) NMDAR1 staining in the squamous epithelium (as the arrow pointed) was slightly positive in a few cases of the MT group. IMT, immature teratoma; MT, mature teratoma; NMDAR, N-methyl-D-aspartate receptor.

Article Snippet: The main antibodies (Abs) used were rabbit anti-human CD4 monoclonal antibody (dilution 1:100, Boster, China), rabbit anti-human CD20 polyclonal antibody (dilution 1:100, Boster, China), rabbit anti-human NMDAR2A antibody (dilution 1:100, Boster, China), rabbit anti-human NMDAR2B antibody (dilution 1:100, Tri-Eagle, China), rabbit anti-human NMDAR 1 antibody (dilution 1:100, Boster, China).

Techniques: Expressing, Staining